Rapid qualitative method for detecting staphylococcal nuclease in foods
نویسندگان
چکیده
منابع مشابه
Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity.
Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.
متن کاملAn improved method for the purification of staphylococcal nuclease.
Commercial staphylococcal nuclease (Foggi strain) containing small amounts of contaminating protein can be purified by chromatography on phosphorylated cellulose columns. The purified enzyme is free of acid and alkaline phosphatase activities. It is homogeneous by polyacrylamide gel electroptfresis, and antigenically pure by immunodiffusion. The E” 1 Cm of the pure enzyme at neutral pH is 9.3 a...
متن کامل[Cold denaturation of staphylococcal nuclease].
It has been shown that the structure of staphylococcal nuclease breaks down reversibly both at a temperature increase above 20 degrees C and at its decrease. Both the heat and cold denaturations of protein are well approximated by a transition between two states differing in heat capacity, which means that the whole protein molecule represents a unique cooperative system with a well developed h...
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[This corrects the article on p. 223 in vol. 40.][This corrects the article on p. 225 in vol. 40.][This corrects the article on p. 226 in vol. 40.][This corrects the article on p. 229 in vol. 40.].
متن کاملStaphylococcal nuclease: sequential assignments and solution structure.
Sequential assignments are reported for backbone 15N and 1H of nearly all residues of staphylococcal nuclease (Nase) complexed with thymidine 3',5'-diphosphate and Ca2+. Because of the relatively large size of the Nase ternary complex, Mr 18K, the crucial element of our assignment strategy was the use of isotope-edited two-dimensional NMR spectra, particularly 15N-edited nuclear Overhauser enha...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1978
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.35.6.1193-1197.1978